1ODG
Very-short-patch DNA repair endonuclease bound to its reaction product site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 102.805, 102.805, 64.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.700 - 2.800 |
| R-factor | 0.2873 |
| Rwork | 0.287 |
| R-free | 0.35600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cw0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.070 | 0.384 |
| Number of reflections | 10084 | 1464 * |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 | 14 * | 25 MM HEPES PH7.0, 75 MM NACL, 15% PEG 8000, 50 MM SODIUM CACODYLATE PH 6.5, 75 MM AMMONIUM SULPHATE AND 10% GLYCEROL PROTEIN 2.5 MG/ML |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | HEPES | 25 (mM) | pH7.0 |
| 2 | 1 | 1 | 75 (mM) | ||
| 3 | 1 | 1 | PEG8000 | 15 (%) | |
| 4 | 1 | 1 | sodium cacodylate | 50 (mM) | pH6.5 |
| 5 | 1 | 1 | ammonium sulfate | 75 (mM) | |
| 6 | 1 | 1 | glycerol | 10 (%) | |
| 7 | 1 | 1 | protein | 2.5 (mg/ml) |
