1ODF

Structure of YGR205w protein.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	64.850, 64.850, 140.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.250
R-factor	0.2063
Rwork	0.206
R-free	0.26000 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.195 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE/RESOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.055	0.499
Total number of observations	94854 *
Number of reflections	14707
<I/σ(I)>	33.2	3.9
Completeness [%]	98.9	98.4
Redundancy	6.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	2.4M AMMONIUM SULFATE, 0.1M NA CITRATE PH5.6, pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)	pH8
3	1	reservoir	ammonium sulfate	1.9 (M)
4	1	reservoir	sodium acetate	0.1 (M)	pH4.5