1ODE
Crystal Analysis Of Chorismate Mutase From Thermus Thermophilus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-15 |
| Detector | RIGAKU IMAGE PLATE RAXIS V |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.319, 78.540, 86.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.020 - 1.650 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.064 | 0.383 |
| Number of reflections | 174516 | |
| <I/σ(I)> | 23.5 | 2.4 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.4 | PROTEIN WAS CRYSTALLIZED USING MICRO BATCH METHOD UNDER OIL FROM 27.5% PEG 4000, 10% DIOXANE AND 100 MM, TRIS/HCL(PH 8.4). |
