1ODD

OMPR C-TERMINAL DOMAIN (OMPR-C) FROM ESCHERICHIA COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	FUJI
Spacegroup name	P 32 2 1
Unit cell lengths	59.160, 59.160, 58.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.226
Rwork	0.224
R-free	0.29600
Structure solution method	MAD WITH SE-MET MUTANT
RMSD bond length	0.010 *
RMSD bond angle	1.560 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.072	0.307
Total number of observations	31412 *
Number of reflections	6153
<I/σ(I)>	8	1.9
Completeness [%]	98.1	96.3
Redundancy	5.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	18 *	Kondo, H., (1994) J.Mol.Biol., 235, 780. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	citric buffer	20 (mM)
2	1	reservoir	ammonium sulfate	0.5 (M)