1OD8
Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-isofagomine lactam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.814, 45.878, 85.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.050 |
| R-factor | 0.104 |
| Rwork | 0.103 |
| R-free | 0.12600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e0x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.689 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.03) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.062 | 0.441 |
| Number of reflections | 121638 | |
| <I/σ(I)> | 26.4 | 2.3 |
| Completeness [%] | 98.0 | 86.4 |
| Redundancy | 4.7 | 2.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20 MG/ML PROTEIN, 16% PEG 5K MME, 100 MM IMIDAZOLE, PH 7.5, 5% ISOPROPANOL |
