1OD5

Crystal structure of glycinin A3B4 subunit homohexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1999-05-15
Detector	SIEMENS
Spacegroup name	H 3
Unit cell lengths	114.842, 114.842, 191.572
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.201
Rwork	0.201
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fxz
RMSD bond length	0.013
RMSD bond angle	3.100
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.300	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.080 *	0.447
Total number of observations	227710 *
Number of reflections	55186	5597 *
<I/σ(I)>	0.08	1.92
Completeness [%]	100.0	100
Redundancy	4.13	2.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4 *	20 *	pH 5.40

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	MES		pH4.0
3	1	2	MPD