1OD4

Acetyl-CoA Carboxylase Carboxyltransferase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2002-05-02
Spacegroup name	C 1 2 1
Unit cell lengths	246.910, 123.910, 145.070
Unit cell angles	90.00, 94.11, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.226
Rwork	0.226
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	23.200 *
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.076	0.181
Number of reflections	533916 *
<I/σ(I)>	16	7
Completeness [%]	98.0	93
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *	4 *	pH 5.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)	pH5.5
2	1	reservoir	PEG8000	10 (%(w/v))
3	1	reservoir	glycerol	5 (%(v/v))
4	1	drop	protein	7 (mg/ml)
5	1	drop	acetyl-CoA	1 (mM)