1OD3
Structure of CSCBM6-3 From Clostridium stercorarium in complex with laminaribiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.183, 52.154, 64.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.000 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.14600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nae |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.003 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.060 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.053 | 0.409 |
| Number of reflections | 61302 | |
| <I/σ(I)> | 17.8 | 3.3 |
| Completeness [%] | 97.2 | 94.2 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 26 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
