1OD2
Acetyl-CoA Carboxylase Carboxyltransferase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Collection date | 2002-07-12 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.870, 138.130, 101.400 |
| Unit cell angles | 90.00, 114.42, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.700 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.052 | 0.116 |
| Number of reflections | 168936 | |
| <I/σ(I)> | 14 | 7 |
| Completeness [%] | 92.0 | 81 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 2 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 3 | 1 | reservoir | glycerol | 5 (%(v/v)) | |
| 4 | 1 | drop | protein | 7 (mg/ml) | |
| 5 | 1 | drop | acetyl-CoA | 1 (mM) |
