1OD0
Family 1 b-glucosidase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.313, 94.547, 113.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.110 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qox |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.171 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.03) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.392 |
| Number of reflections | 58694 | |
| <I/σ(I)> | 19.6 | 3.44 |
| Completeness [%] | 99.3 | 98.1 |
| Redundancy | 3.9 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10MG/ML PROTEIN IN 15% PEG 4K, 0.2 M CA ACETATE, 0.1M IMIDAZOLE PH 7.0 |
