1OCT

CRYSTAL STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE: DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	C 2 2 21
Unit cell lengths	97.500, 89.800, 80.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.237
Rwork	0.237
RMSD bond length	0.006 *
RMSD bond angle	1.200 *
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.065 *
Total number of observations	22955 *
Number of reflections	6998 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	PEG3350	30-34 (%)
4	1	drop	ammonium sulphate	0.10-0.15 (M)
5	1	drop	citrate	0.05 (M)
6	1	reservoir	citrate	0.05 (M)