Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAC Science DIP-2030 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.287, 95.581, 74.899 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22600 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.276 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 68327 | |
<I/σ(I)> | 41.76 | 9.81 |
Completeness [%] | 97.4 | 93.4 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% POLYETHYLENE GLYCOL 1000, 100 MM TRIS, PH 7.0 |