1OBY
Crystal structure of the complex of PDZ2 of syntenin with a syndecan-4 peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-31 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.337, 54.441, 50.219 |
| Unit cell angles | 90.00, 98.65, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.850 |
| Rwork | 0.175 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n99 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.820 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.049 | 0.413 |
| Total number of observations | 46421 * | |
| Number of reflections | 12987 * | 1108 * |
| <I/σ(I)> | 25.8 | 2.86 |
| Completeness [%] | 97.4 | 83.7 |
| Redundancy | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 294 * | SITTING DROP WITH 0.1 M HEPES, PH 6.8, 1.6 M AMMONIUM SULFATE, 20 MM COCL2, AND 0.2 MGSO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.1 (M) | pH6.8 |
| 2 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | 0.2 (M) |
