1OBT

STRUCTURE OF RICIN A CHAIN MUTANT, COMPLEX WITH AMP

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1996-02-16
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 41 21 2
Unit cell lengths	68.650, 68.650, 141.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.197
Rwork	0.197
RMSD bond length	0.012
RMSD bond angle	25.530 *
Data reduction software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Rmerge	0.069
Number of reflections	23545
Completeness [%]	99.7
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop	phosphate	3 (mM)
3	1	drop	sodium chloride	100 (mM)
4	1	drop	ammonium sulfate	0.2 (M)
5	1	drop	sodium acetate	0.03 (M)
6	1	reservoir	ammonium sulfate	0.5 (M)
7	1	reservoir	sodium acetate	0.1 (M)