1OBQ
Apocrustacyanin C1 crystals grown in space and earth using vapour diffusion geometry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.959, 80.539, 110.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.550 - 1.850 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.028 * |
| RMSD bond angle | 2.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.550 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 28666 |
| Completeness [%] | 86.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | Snell, E.H., (1997) Acta Crystallogr.,Sect.D, 53, 231. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | EDTA | 1 (mM) | pH7.0 |
| 4 | 1 | reservoir | MPD | 5 (%(v/v)) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH9.0 |
| 7 | 1 | reservoir | ammonium sulfate | 1.9 (M) |
