Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1OBO

W57L flavodoxin from Anabaena

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineX11
Temperature [K]100
Detector technologyCCD
Collection date2002-11-15
DetectorMARRESEARCH
Spacegroup nameP 1 21 1
Unit cell lengths37.630, 73.100, 57.050
Unit cell angles90.00, 102.58, 90.00
Refinement procedure
Resolution20.000

*

- 1.200
R-factor0.186
Rwork0.186
R-free0.20300

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1flv
RMSD bond length0.005
RMSD bond angle22.900

*

Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.260
High resolution limit [Å]1.2001.200
Rmerge0.038

*

0.139

*

Total number of observations200831

*

Number of reflections81435

*

11496

*

<I/σ(I)>10.83.8
Completeness [%]94.792.1
Redundancy3.63.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

*

6295

*

pH 6.00
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21reservoirammonium sulfate3.5 (M)
31reservoirMES0.1 (M)pH6.0

227344

PDB entries from 2024-11-13

PDB statisticsPDBj update infoContact PDBjnumon