1OBJ
Crystal structure of the T150A mutant of Malonamidase E2 from Bradyrhizobium japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.941, 94.326, 74.741 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.22900 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | 1GR8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 58083 | |
| <I/σ(I)> | 13 | 2.68 |
| Completeness [%] | 91.7 | 81.3 |
| Redundancy | 4.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% POLYETHYLENE GLYCOL, 0.1 M TRIS-HCL, PH 7.0 |
