1OBJ
Crystal structure of the T150A mutant of Malonamidase E2 from Bradyrhizobium japonicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 102.941, 94.326, 74.741 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.179 |
Rwork | 0.179 |
R-free | 0.22900 |
Structure solution method | DIRECT METHODS |
Starting model (for MR) | 1GR8 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.256 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 58083 | |
<I/σ(I)> | 13 | 2.68 |
Completeness [%] | 91.7 | 81.3 |
Redundancy | 4.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% POLYETHYLENE GLYCOL, 0.1 M TRIS-HCL, PH 7.0 |