1OBF
The crystal structure of Glyceraldehyde 3-phosphate Dehydrogenase from Alcaligenes xylosoxidans at 1.7A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.984, 146.622, 146.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.16701 |
Rwork | 0.166 |
R-free | 0.18900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gd1 |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.497 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.099 | 0.350 * |
Number of reflections | 104690 * | |
<I/σ(I)> | 22 | 2.2 |
Completeness [%] | 97.6 | 90.7 |
Redundancy | 6. * | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | PROTEIN 5MG/ML, 12 % PEG550, 100 MM POTASSIUM THIOCYANATE, 50 MM MES BUFFER (PH 6.5) |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | PEG550 | 12 (%) | |
3 | 1 | drop | potassium thiocyanate | 100 (mM) | |
4 | 1 | drop | MES | 50 (mM) | pH6.5 |
5 | 1 | reservoir | PEG550 | 24.5 (%) | |
6 | 1 | reservoir | potassium thiocyanate | 200 (mM) | |
7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |