1OBF
The crystal structure of Glyceraldehyde 3-phosphate Dehydrogenase from Alcaligenes xylosoxidans at 1.7A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.984, 146.622, 146.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.16701 |
| Rwork | 0.166 |
| R-free | 0.18900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd1 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.497 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.099 | 0.350 * |
| Number of reflections | 104690 * | |
| <I/σ(I)> | 22 | 2.2 |
| Completeness [%] | 97.6 | 90.7 |
| Redundancy | 6. * | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | PROTEIN 5MG/ML, 12 % PEG550, 100 MM POTASSIUM THIOCYANATE, 50 MM MES BUFFER (PH 6.5) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG550 | 12 (%) | |
| 3 | 1 | drop | potassium thiocyanate | 100 (mM) | |
| 4 | 1 | drop | MES | 50 (mM) | pH6.5 |
| 5 | 1 | reservoir | PEG550 | 24.5 (%) | |
| 6 | 1 | reservoir | potassium thiocyanate | 200 (mM) | |
| 7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
