1OBB
alpha-glucosidase A, AglA, from Thermotoga maritima in complex with maltose and NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.000, 85.700, 83.600 |
| Unit cell angles | 90.00, 106.00, 90.00 |
Refinement procedure
| Resolution | 17.650 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23400 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.530 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 * | 0.362 * |
| Number of reflections | 77496 | |
| <I/σ(I)> | 16.9 | 2.4 |
| Completeness [%] | 96.6 * | 92.6 * |
| Redundancy | 2.5 * | 2.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 10% PEG 6000, 1M LICL, 0.1M TRIS/HCL PH4.6, 2MM MNCL2, 50MM MALTOSE, 50MM NAD+, pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
| 4 | 1 | reservoir | 1 (M) | ||
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH4.6 |
| 6 | 1 | reservoir | 2 (mM) |
