1OB8

Holliday Junction Resolving Enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-04
Detector	ADSC CCD
Spacegroup name	P 65
Unit cell lengths	90.577, 90.577, 70.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.540 - 1.800
Rwork	0.197
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SE-SAD DERIVED STRUCTURE
RMSD bond length	0.010
RMSD bond angle	1.292
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	30127
<I/σ(I)>	41.1	1.75
Completeness [%]	98.0	94.1
Redundancy	4.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	293	0.2M LITHIUM SULFATE,0.1M TRIS PH 9 20% PEG 4000,15% ETHYLENE GLYCOL,293K