1OB3
Structure of P. falciparum PfPK5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 222.088, 44.267, 63.044 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 36.200 * - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.094 | 0.414 |
| Total number of observations | 120555 * | |
| Number of reflections | 46965 | |
| <I/σ(I)> | 1.6 | 1.6 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 100MM HEPES SODIUM SALT PH 7.5, 10MM MGCL2, PEG 8K (8-16%), ISOPROPANOL (2-8%) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | HEPES | 40 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | MTG | 0.02 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 8-16 (%) | |
| 8 | 1 | reservoir | isopropanol | 2-8 (%) |
