1OB2
E. coli elongation factor EF-Tu complexed with the antibiotic kirromycin, a GTP analog, and Phe-tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 196.410, 196.410, 196.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.640 - 3.350 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ttt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | Agrovata |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.530 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.154 | 0.429 |
| Number of reflections | 18794 | |
| <I/σ(I)> | 10.1 | 3 |
| Completeness [%] | 98.5 | 99.5 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 1.8M AMMONIUM SULPHATE, 10 MM MAGNESIUM CHLORIDE, 20 MM TRIS-MES, PH 6.4 |
