1OAY
Antibody multispecificity mediated by conformational diversity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.174, 79.142, 168.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 * |
R-factor | 0.226 |
Rwork | 0.258 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ANQ |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.500 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.268 | 2.810 |
High resolution limit [Å] | 2.400 * | 2.660 |
Rmerge | 0.126 * | 0.583 * |
Number of reflections | 47809 * | |
<I/σ(I)> | 6.6 | 3.1 |
Completeness [%] | 99.2 * | 94.7 * |
Redundancy | 6.7 * | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 * | 0 22% PEG 8K 0.1 M SODIUM CACODYLATE, 0.2M SODIUM ACETATE, PH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 22 (%) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
3 | 1 | reservoir | sodium acetate | 0.2 (M) | pH5.0 |