1OAU

Fv Structure of the IgE SPE-7 in complex with DNP-Ser (immunising hapten)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-09-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.459, 79.480, 168.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.020 - 1.800
R-factor	0.226
Rwork	0.226
R-free	0.26900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ANQ
RMSD bond length	0.008 *
RMSD bond angle	1.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.015	1.900
High resolution limit [Å]	1.800 *	1.810
Rmerge	0.070 *	0.235 *
Number of reflections	90267 *
<I/σ(I)>	10.9	1.2
Completeness [%]	91.8	91.4 *
Redundancy	3.0 *	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *		24% PEG8K,0.1M NA ACETATE, 0.2M AMMONIUM SULPHATE, PH7, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	25 (%)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	ammonium sulfate	0.2 (M)	pH5.6