1OAR

Fv IgE SPE-7 in complex with Alizarin Red

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-11-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	78.917, 78.882, 169.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 2.200 *
Rwork	0.205
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ANQ
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.800
High resolution limit [Å]	2.200 *
Rmerge	0.067	0.325 *
Number of reflections	52239 *
Completeness [%]	99.6	99.4 *
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *		21% PEG 8K, 0.1M NA CACODYLATE, 0.2M NA ACETATE PH5.5, pH 5.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	21 (%)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir	sodium acetate	0.2 (M)	pH5.5