1OAQ
Free conformation Ab1 of the IgE SPE-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.541, 70.743, 82.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.330 - 1.500 |
| R-factor | 0.15965 |
| Rwork | 0.160 |
| R-free | 0.18200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ANQ |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 * | 0.238 * |
| Number of reflections | 34284 * | |
| <I/σ(I)> | 9.3 | 3.6 |
| Completeness [%] | 92.8 * | 92.8 * |
| Redundancy | 12.6 * | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | 1.2M NA CITRATE 0.2M NA CACODYLATE PH5, 0.2M LITHIUM, pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 1 (M) | pH6.2 |
