1OAK
CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN IN COMPLEX WITH THE FUNCTION BLOCKING NMC-4 FAB
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.200, 61.900, 72.900 |
| Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT AND MULTIPLE ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | NMC4 (FAB) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.184 | |
| Number of reflections | 44346 | |
| <I/σ(I)> | 18.1 | 6.4 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
