1OAG

Ascorbate peroxidase from soybean cytosol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-13
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	81.842, 81.842, 75.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.740 - 1.750
R-factor	0.14375
Rwork	0.142
R-free	0.18200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1apx
RMSD bond length	0.020 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.740 *	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.067	0.320
Total number of observations	92501 *
Number of reflections	26092
<I/σ(I)>	16.1	2.4
Completeness [%]	99.3	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.3		2.25M LI2SO4, 0.1M HEPES, PH8.3, pH 8.30

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	lithium sulfate	2.25 (M)
3	1	reservoir	HEPES	0.1 (M)	pH8.3