1OA7
Structure of Melanocarpus albomyces endoglucanase in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 47.300, 47.300, 178.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eng |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 * | 0.408 |
Total number of observations | 133688 * | |
Number of reflections | 13447 * | |
<I/σ(I)> | 12.1 | 3.6 |
Completeness [%] | 92.4 | 89.1 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.6 | Hirvonen, M., (2002) Acta Cryst., D58, 336. * |