1O9W
F17-aG lectin domain from Escherichia coli in complex with N-acetyl-glucosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 42.329, 42.329, 268.714 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.770 - 1.650 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o9v |
RMSD bond length | 0.011 |
RMSD bond angle | 26.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.650 | 1.660 |
Number of reflections | 20396 | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 30% PEG4000, 0.1M SODIUM ACETATE (PH 4.6), 0.2M AMMONIUM ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 20 (mM) | pH8. |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
5 | 1 | reservoir | ammonium acetate | 0.2 (M) |