1O9V
F17-aG lectin domain from Escherichia coli in complex with a selenium carbohydrate derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9611,0.9792,0.9788 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 42.757, 42.757, 273.758 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 16346 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 97.1 | 93.1 |
| Redundancy | 58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 30% PEG4000, 0.1M SODIUM ACETATE (PH 4.6),0.2M AMMONIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH8. |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | reservoir | ammonium acetate | 0.2 (M) |
