1O9G
rRNA methyltransferase aviRa from Streptomyces viridochromogenes at 1.5A
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Collection date | 2002-09-08 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.362, 47.320, 63.385 |
Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
Resolution | 25.000 * - 1.500 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.20200 |
Structure solution method | MAD |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 * | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.039 * | 0.200 * |
Total number of observations | 289006 * | |
Number of reflections | 64711 | 1482 * |
<I/σ(I)> | 38.7 | 6.6 |
Completeness [%] | 97.0 | 90 |
Redundancy | 4.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.9 | 20 * | PEG 20000 9%, MES 6.6, pH 6.90 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | pH6.6-6.9 |
3 | 1 | reservoir | PEG20000 | 6-8 (%(w/v)) |