1O9F
Structural view of a fungal toxin acting on a 14-3-3 regulatory complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Collection date | 2001-12-15 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 108.800, 108.800, 135.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 * - 2.700 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a4o |
RMSD bond length | 0.008 * |
RMSD bond angle | 18.600 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.300 |
Total number of observations | 56531 * | |
Number of reflections | 13170 | |
<I/σ(I)> | 14 | 4.2 |
Completeness [%] | 96.8 | 97.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.7 * | PEG400, CITRAT PH 4.7, 0.2 MM AMMONIUM ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG400 | 21 (%) | |
2 | 1 | reservoir | citrate | 0.1 (mM) | pH4.7 |
3 | 1 | reservoir | ammonium acetate | 0.2 (mM) | pH7.0 |
4 | 1 | reservoir | dithiothreitol | 10 (mM) |