1O9D
Structural view of a fungal toxin acting on a 14-3-3 regulatory complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2001-12-15 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 110.126, 110.126, 136.464 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 * - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4o |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 19.700 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 | 0.351 |
| Total number of observations | 131256 * | |
| Number of reflections | 21328 | |
| <I/σ(I)> | 18.1 | 4 |
| Completeness [%] | 95.5 | 98.1 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.7 * | PEG400, CITRATE PH 4.7, 0.2 MM AMMONIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 21 (%) | |
| 2 | 1 | reservoir | citrate | 0.1 (mM) | pH4.7 |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (mM) | pH7.0 |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
