1O9C

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2001-11
Spacegroup name	P 65 2 2
Unit cell lengths	109.231, 109.231, 135.865
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a4o
RMSD bond length	0.007
RMSD bond angle	17.000 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.056	0.354
Total number of observations	205883 *
Number of reflections	15163
<I/σ(I)>	33.1	8.2
Completeness [%]	99.1	99.4
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7 *		PEG400, CITRATE PH 4.7, 0.2 MM AMMONIUM ACETATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	21 (%)
2	1	reservoir	citrate	0.1 (mM)	pH4.7
3	1	reservoir	ammonium acetate	0.2 (mM)	pH7.0
4	1	reservoir	dithiothreitol	10 (mM)