1O91
Crystal Structure of a Collagen VIII NC1 Domain Trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.590, 82.550, 89.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gr3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.240 |
| Number of reflections | 37514 * | |
| <I/σ(I)> | 15.7 | 6.7 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.5 * | 1.7 M AMMONIUM SULFATE, 4 % (V/V)ISOPROPANOL, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | glycine-NaOH | 0.1 (M) | pH9.5 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | CHAPS | 0.05 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.7-2.0 (M) | |
| 6 | 1 | reservoir | isopropanol | 4 (%) |
