1O8U
The 2 Angstrom Structure of 6-Oxo Camphor Hydrolase: New Structural Diversity in the Crotonase Superfamily
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2001-09-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97900,0.97937,0.88560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.950, 130.410, 81.320 |
| Unit cell angles | 90.00, 114.16, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.19000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.157 |
| Number of reflections | 95532 | |
| <I/σ(I)> | 25.8 | 7.1 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 2.4 * | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | VAPOUR DIFFUSION HANGING DROP PROTEIN SOLUTION: 10 MG/ML IN 50 MM TRIS-HCL PH 7.0, 1 MM DTT, 20 MICROMOLAR PMSF RESERVOIR: 0.1 M SODIUM ACETATE,0.2 M AMMONIUM SULPHATE, 28% (V/V) PEG 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | PMSF | 20000 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG4000 | 28 (%(v/v)) |
