1O8S
Structure of CsCBM6-3 from Clostridium stercorarium in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.090, 52.069, 64.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.350* |
| R-factor | 0.124 |
| Rwork | 0.123 |
| R-free | 0.14900 |
| Structure solution method | MAD |
| Starting model (for MR) | 1gmm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.799 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.210 |
| High resolution limit [Å] | 1.350 * | 1.350 * |
| Rmerge | 0.072 | 0.162 * |
| Number of reflections | 66726 | |
| <I/σ(I)> | 15.6 | 2.8 |
| Completeness [%] | 97.8 | 86.5 * |
| Redundancy | 3.7 | 3.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 26 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
