1O8Q
The active site of the molybdenum cofactor biosenthetic protein domain Cnx1G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 174.610, 174.610, 174.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.600 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eav |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.389 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.064 | 0.208 |
| Number of reflections | 53034 | |
| <I/σ(I)> | 7.4 | 4.3 |
| Completeness [%] | 97.2 | 90.6 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.7 | Schwarz, G., (2001) J. Mol. Biol., 312, 405. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 2.4 (M) |
