1O8O
The active site of the molybdenum cofactor biosynthetic protein domain Cnx1G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-23 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 122.263, 122.263, 174.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.700 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| Starting model (for MR) | 1eav |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.374 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.570 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.031 | 0.112 |
| Number of reflections | 18549 | |
| <I/σ(I)> | 17.7 | 6.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.4 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5 |
