1O8N
The active site of the molybdenum cofactor biosynthetic protein domain Cnx1G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 122.486, 122.486, 174.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.710 * - 2.800 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eav |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.490 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.157 |
| Number of reflections | 16631 * | |
| <I/σ(I)> | 6.6 | 4.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | Schwarz, G., (2001) J. Mol. Biol., 312, 405. * |
