1O8H

Pectate Lyase C from Erwinia Chrysanthemi at pH 9.5 with 0.3mM Ca2+ Added

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RT3000
Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	71.990, 78.270, 95.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.2061
Rwork	0.206
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1air
RMSD bond length	0.005
RMSD bond angle	25.260 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.200
Rmerge	0.042
Total number of observations	108490 *
Number of reflections	35364
<I/σ(I)>	12.7
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.9 *	4 *	Yoder, M. D., (1990) J. Biol. Chem., 265, 11429. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (A280/ml)
2	1	drop	ammonium sulfate	1.0 (M)
3	1	drop	HEPES	50 (mM)
4	1	drop		1 (mM)
5	1	reservoir	ammonium sulfate	2.0 (M)
6	1	reservoir	HEPES	0.1 (M)