1O8E
Pectate Lyase C from Erwinia Chrysanthemi at pH 11.2 with 1mM Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RT3000 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.480, 76.760, 91.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.2122 |
| Rwork | 0.212 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1air |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.350 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.076 |
| Total number of observations | 99422 * |
| Number of reflections | 27422 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 92.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.9 * | 4 * | Yoder, M. D., (1990) J. Biol. Chem., 265, 11429. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (A280/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | drop | HEPES | 50 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
