1O8C
CRYSTAL STRUCTURE OF E. COLI K-12 YHDH WITH BOUND NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 189.313, 189.313, 98.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 158.110 - 2.600 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE YHD PDB ID CODE 1O89 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.192 |
| Number of reflections | 62012 | |
| <I/σ(I)> | 8.3 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 8.5 % PEG 8K, 0.1 M NA-ACETATE PH 5.5, 0.01 MM ZNCL2, 5 MM NADPH |
