1O8B
Structure of Escherichia coli ribose-5-phosphate isomerase, RpiA, complexed with arabinose-5-phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.049, 42.400, 60.195 |
| Unit cell angles | 90.23, 100.98, 98.98 |
Refinement procedure
| Resolution | 40.000 - 1.250 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ks2 |
| RMSD bond length | 0.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 90067 | |
| <I/σ(I)> | 12.4 | 1.1 |
| Completeness [%] | 81.8 | 40.1 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | CRYSTALS WERE GROWN FROM A SOLUTION CONTAINING 3.5 MG/ML PROTEIN, 30-35 % PEG 4000 10 MM ARABINOSE-5-PHOSPHATE, 0.05M TRIS-HCL PH 8.4, 0.1 M MGCL2 |
