1O87
A new MgGDP complex of the Ffh NG domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-06-14 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.415, 113.631, 61.382 |
Unit cell angles | 90.00, 111.76, 90.00 |
Refinement procedure
Resolution | 56.800 - 2.100 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23600 * |
Structure solution method | MAD |
Starting model (for MR) | 1ffh |
RMSD bond length | 0.018 |
RMSD bond angle | 1.669 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.045 | 0.159 |
Number of reflections | 40999 | |
<I/σ(I)> | 24.1 | 6.1 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 4.0 M SODIUM FORMATE, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 32 (mg/ml) | |
2 | 1 | drop | 2 (mM) | ||
3 | 1 | drop | GTP | 2 (mM) | |
4 | 1 | reservoir | sodium formate | 4 (M) |