1O84
Crystal Structure of Bacteriocin AS-48. N-decyl-beta-D-maltoside Bound.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF NONIUS |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.488, 76.488, 51.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.23669 |
Rwork | 0.236 |
R-free | 0.25600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 * |
RMSD bond angle | 2.141 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.300 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.138 | 0.591 * |
Number of reflections | 4544 | |
<I/σ(I)> | 5.1 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.1 | 9.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | CRYSTALS WERE GROWN USING VAPOUR DIFFUSION TECHNIQUES FROM DROPS CONTAINING AS48 (20 MG/ML), 18 MM N-DECYL-BETA-D-MALTOSIDE AND RESERVOIR SOLUTION (0.2 M AMMONIUM SULPHATE,25 % W/V POLYETHYLENE GLYCOL 4000) IN A RATIO 4:1:5, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | PEG4000 | 25 (%(w/v)) |