1O7Z

Crystal structure of IP-10 T-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-25
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	59.437, 59.437, 121.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 * - 1.920
R-factor	0.279
Rwork	0.279
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rhp
RMSD bond length	0.006
RMSD bond angle	25.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.087	0.474
Total number of observations	309571 *
Number of reflections	17435
<I/σ(I)>	21.7	3.2
Completeness [%]	99.8	100
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.75	16 *	10MG/ML PROTEIN,0.1M TRIS-HCL BUFFER, PH 8.75,3.3M SODIUM FORMATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.75
3	1	reservoir	sodium formate	3.3 (M)