1O7Z
Crystal structure of IP-10 T-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.437, 59.437, 121.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 1.920 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rhp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.087 | 0.474 |
| Total number of observations | 309571 * | |
| Number of reflections | 17435 | |
| <I/σ(I)> | 21.7 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.75 | 16 * | 10MG/ML PROTEIN,0.1M TRIS-HCL BUFFER, PH 8.75,3.3M SODIUM FORMATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.75 |
| 3 | 1 | reservoir | sodium formate | 3.3 (M) |
