1O7T
Metal nanoclusters bound to the Ferric Binding Protein from Neisseria gonorrhoeae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 148.128, 148.128, 115.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 * |
| R-factor | 0.1642 |
| Rwork | 0.164 |
| R-free | 0.26400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.584 |
| Number of reflections | 406392 | |
| <I/σ(I)> | 7.4 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 290 | 4MG/ML PROTEIN IN 4MM PHOSPHATE, 25MM NAHCO3 PLUS 20% PEG 4000, 0.2M KCL, 0.4M IMIDAZOLE/MALATE BUFFER PH7.7, pH 7.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | imidazole-malate | 0.4 (M) | pH7.7 |
