1O7I

Crystal structure of a single stranded DNA binding protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	130
Detector technology	CCD
Collection date	2002-03-15
Detector	ADSC CCD
Wavelength(s)	0.9600, 0.9780, 0.9786
Spacegroup name	P 61
Unit cell lengths	75.805, 75.805, 70.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.000 - 1.260 *
R-factor	0.193
Rwork	0.191
R-free	0.21000 *
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920 *	1.730
High resolution limit [Å]	1.260 *	1.260 *
Rmerge	0.081 *	0.352
Total number of observations	463924 *
Number of reflections	60733 *
<I/σ(I)>	8.1	2.2
Completeness [%]	99.9 *	99.9 *
Redundancy	7.5 *	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Kerr, I.D., (2001) Acta Crystallogr.,Sect.D, 57, 1290. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	ammonium sulfate	2.4 (M)
3	1	reservoir	citric acid	100 (mM)	pH3.5-5.0